Классификация USP L ВЭЖХ колонок Phenomenex
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For each United States Pharmacopeia (USP) column specification, It is widely understood that all HPLC packings are not alike, and no The USP does give chromatographers the flexibility to make adjustments |
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| USP | Recommended Column | Shape | |
| L1 | Octadecyl silane chemically bonded to porous or non-porous silica or ceramic microparticles, 1.5 to 10 μm in diameter, or a monolithic rod. |
Kinetex® C18 Kinetex EVO C18 Kinetex Polar C18 Kinetex PS C18 Kinetex XB-C18 Luna® C18(2) Luna Omega C18 Luna Omega PS C18 Luna Omega Polar C18 Gemini® NX-C18 Gemini C18 Synergi™ Hydro-RP Synergi Fusion-RP bioZen™ Peptide PS-C18 Onyx™ C18 Jupiter® C18 Clarity® Oligo-RP Clarity Oligo-MS Clarity Oligo-XT Aeris™ WIDEPORE XB-C18 bioZen Peptide XB-C18 |
Core-Shell Core-Shell Core-Shell Core-Shell Core-Shell Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Spherical Monolith Spherical Spherical Core-Shell Core-Shell Core-Shell Core-Shell |
| L3 | Porous silica particles, 1.5 to 10 μm in diameter, or a monolithic silica rod. | Kinetex HILIC Luna Silica(2) Onyx Silica |
Core-Shell Spherical Monolith |
| L7 | Octyl silane chemically bonded to totally or superficially porous silica particles, 1.5 to 10 μm in diameter, or a monolithic silica rod. |
Kinetex C8 Luna C8(2) Onyx C8 bioZen Intact XB-C8 |
Core-Shell Spherical Monolith Core-Shell |
| L8 | An essentially monomolecular layer of aminopropyl-silane chemically bonded to totally porous silica gel support, 1.5 to 10 μm in diameter, or a monolithic silica rod. |
Luna NH2 Luna Omega SUGAR |
Spherical Spherical |
| L9 | Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic cation-exchange coating, 3 to 10 µm in diameter. |
Luna SCX | Spherical |
| L10 | Nitrile groups chemically bonded to porous silica particles, 1.5 to 10 μm in diameter, or a monolithic silica rod. |
Luna CN | Spherical |
| L11 | Phenyl groups chemically bonded to porous silica particles, 1.5 to 10 μm in diameter, or a monolithic silica rod. |
Kinetex Biphenyl Kinetex Phenyl-Hexyl Synergi Polar-RP Luna Phenyl-Hexyl Gemini C6-Phenyl Prodigy PH-3 |
Core-Shell Core-Shell Spherical Spherical Spherical Spherical |
| L13 | Trimethylsilane chemically bonded to porous silica particles, 3 to 10 μm in diameter. | Develosil® TMS-UG (C1) 130 Å | Spherical |
| L14 | Silica gel having a chemically bonded, strongly basic quaternary ammonium anion-exchange coating, 5 to 10 µm in diameter. |
PhenoSphere™ SAX | Spherical |
| L15 | Hexyl silane chemically bonded to totally porous silica particles, 3 to 10 μm in diameter. | PhenoSphere C6 | Spherical |
| L16 | Dimethyl silane chemically bonded to porous silica particles, 5 to 10 μm in diameter. | ||
| L17 | Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 6 to 12 μm in diameter. |
Rezex RHM-Monosaccharide | Spherical |
| L19 | Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the calcium form, 5 to 15 μm in diameter. |
Rezex RCM-Monosaccharide Rezex RCU-Sugar Alcohols |
Spherical Spherical |
| L20 | Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5 to 10 μm in diameter, or a monolithic silica rod. |
Luna HILIC BioSep™-SEC-S Yarra™ SEC |
Spherical Spherical Spherical |
| L21 | A rigid, spherical styrene-divinylbenzene copolymer, 3 to 30 μm in diameter. | PolymerX™ RP-1 Phenogel™ 100 Å |
Spherical Spherical |
| L22 | A cation-exchange resin made of porous polystyrene gel with sulfonic acid groups, 5 to 15 μm in diameter. |
Rezex ROA-Organic Acid | Spherical |
| L23 | An anion-exchange resin made of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups, 7-12 μm in size. |
Shodex® IEC QA-825 | Spherical |
| L25 | Packing having the capacity to separate compounds with a MW range from 100 to 5000 daltons (as determined by polyethylene oxide), applied to neutral, anionic, and cationic water-soluble polymers.A polymethacrylate resin base, crosslinked with poly-hydroxylated ether (surface contained some residual carboxyl functional groups) was found suitable. |
PolySep™-GFC-P2000 Shodex OHpak SB-802.5HQ |
Spherical Spherical |
| L26 | Butyl silane chemically bonded to totally or superficially porous silica particles, 1.5 to 10 μm in diameter. |
Jupiter 300 C4 bioZen Intact C4 |
Spherical Core-Shell |
| L27 | Porous silica particles, 30 to 50 μm in diameter. | Sepra Silica | Irregular |
| L33 | Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 daltons. It is spherical, silica-based and processed to provide pH stability. |
Yarra SEC-2000 BioSep-SEC-S2000 Yarra SEC-3000 BioSep-SEC-S3000 |
Spherical Spherical Spherical Spherical |
| L34 | Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the lead form, 7 to 9 μm in diameter. |
Rezex RPM-Monosaccharide | Spherical |
| L35 | A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type) molecular monolayer bonded phase having a pore size of 150Å. |
(BioSep-SEC-S2000 or Yarra SEC-2000 may be used) |
Spherical |
| L37 | Polymethacrylate gel packing having the capacity to separate proteins by molecular size over a range of 2,000 to 40,000 daltons. |
PolySep-GFC-P3000 Shodex OHpak SB-803HQ |
Spherical Spherical |
| L38 | Methacrylate-based size-exclusion packing for water-soluble samples. | PolySep-GFC-P series Shodex OHpak SB-800HQ |
Spherical Spherical |
| L39 | Hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin. | PolySep-GFC-P series Shodex OHpak SB-800HQ series Shodex RSpak DM-614 |
Spherical Spherical Spherical |
| L40 | Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles, 3 μm to 20 μm in diameter. |
Lux Cellulose-1 | Spherical |
| L43 | Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 1.5 to 10 μm in diameter. |
Kinetex F5 Luna PFP(2) |
Core-Shell Spherical |
| L45 | Beta cyclodextrin, R, S-hydroxypropyl ether derivative, bonded to porous silica particles, 3 to 10 μm in diameter |
Shiseido Chiral CD-Ph | Spherical |
| L51 | Amylose tris-3,5-dimethylphenylcarbamate-coated, porous, spherical, silica particles, 3 to 10 μm in diameter. |
Lux Amylose-1 | Spherical |
| L57 | A chiral-recognition protein, ovomucoid, chemically bonded to silica particles, about 5 μm in diameter, with a pore size of 120Å. |
Ultron ES-OVM | Spherical |
| L58 | Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium form, about 6 to 30 μm in diameter. |
Rezex RNM-Carbohydrate | Spherical |
| L59 | Size-exclusion separations of proteins (separation by molecular weight) over the range of 5 to 7000 kDa. Spherical (1.5 to 10 μm), silica or hybrid packing with a hydrophilic coating. |
Yarra SEC-2000 BioSep-SEC-S2000 Yarra SEC-3000 BioSep-SEC-S3000 |
Spherical Spherical Spherical Spherical |
| L62 | C30 silane bonded phase on a fully porous spherical silica, 3 to 15 μm in diameter. | Develosil Combi-RP Develosil RP-Aqueous Develosil RP-Aqueous-AR |
Spherical Spherical Spherical |
| L67 | Porous vinyl alcohol copolymer with a C18 alkyl group attached to the hydroxyl group of the polymer, 2 to 10 μm in diameter. |
Asahipak ODP-50 | Spherical |
| L71 | A rigid, spherical polymethacrylate 4 to 6 µm in diameter. | Shodex RSpak DE-413 Shodex RSpak DE-613 |
Spherical Spherical |
| L80 | Cellulose tris(4-methylbenzoate)-coated, porous, spherical, silica particles, 5 to 20 µm in diameter. |
Lux Cellulose-3 | Spherical |
| L82 | Polyamine chemically bonded to cross-linked polyvinyl alcohol polymer, 4 - 5 μm in diameter |
Asahipak NH2P-50 | Spherical |
| L87 | Dodecyl silane chemically bonded to porous silica particles, 1.5 to 10 µm in diameter. | Synergi Max-RP | Spherical |
| L93 | Cellulose tris (3,5-dimethylphenylcarbamate) reversed phase chiral stationary phase coated on 3 or 5 µm silica gel particles. |
Lux Cellulose-1 | Spherical |
| L96 | Alkyl chain, reversed phase bonded to totally or superficially porous silica designed to retain hydrophilic and other polar compounds when using highly aqueous mobile phases, including 100 % aqueous, 1.5 μm to 10 μm in diameter. |
Kinetex Polar C18 Kinetex PS C18 Luna Omega Polar C18 Luna Omega PS C18 Kinetex EVO C18 Synergi Hydro-RP Synergi Fusion-RP |
Core-Shell Core-Shell Spherical Spherical Core-Shell Spherical Spherical |
| L99 | Amylose tris-(3,5- dimethylphenylcarbamate), immobilized on porous, spherical, silica particles, to 5 μm in diameter |
Lux i-Amylose-1 | Spherical |
| L107 | Cellulose tris(4-methylbenzoate)-coated porous spherical particles, 3 to 5 μm in diameter, for use with reversed phase mobile phases. |
Lux Cellulose-3 | Spherical |
| L118 | Aqueous polymerized C18 groups on silica particles, 1.2 to 5 µm in diameter. | Kinetex PAH | Core-Shell |
| L119 | Cellulose tris-(3,5-dichlorophenylcarbamate), immobilized on porous, spherical, silica particles, 3 to 5 µm in diameter. |
Lux i-Cellulose-5 | Spherical |
Данный раздел находится в разработке.
Goals of Sample Preparation
Successful sample preparation for most analytical techniques (HPLC, UPLC, ™LC-MS, UV, GC, etc.)
has a threefold objective. It needs to provide the sample component of interest:
■■ In solution
■■ Free from interfering matrix elements
■■ At a concentration appropriate for detection or measurement
Waters™ Sample Preparation Solutions for quantitative analysis make it easy to deliver a sample that is reproducible with
high recovery and free of interferences. Based on simple, logical workflows that produce clean samples through selective
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