Колонки ВЭЖХ Xbridge

XBridge BEH HPLC Columns are designed for one purpose—to maximize
productivity. Whether you are creating a quality-control method or developing
a leading-edge LC-MS assay, there is an XBridge Column that will fit your
separation needs.

■■Unique, mobile-phase, pH stability, increasing column lifetime
■■Remarkable column reliability, ensuring the ruggedness of assays
■■Exceptional particle efficiency, providing unmatched peak shape and capacity

With 10 general-purpose, application-specific sorbents and the widest range of
particle sizes available, no other HPLC column family offers the tools you need
to meet the most demanding chromatographic challenges. Whether you require
robust HPLC methods, seamless UPLC transferability, or preparative scaling for
product isolation, count on the versatility of an XBridge BEH HPLC Column.

Характеристики сорбентов

	Ligand Density, μmol/m²	Carbon Load	Endcapped	USP Class No.	pH Range	Temperature Limits	Surface Area, m²/g
BEH C18, 130 Å UHPLC: 2.5 μm XP HPLC: 3.5, 5, 10 μm	3.1	18%	Yes	L1	1-12	Low pH = 80 ˚C, High pH = 60 ˚C	185
BEH Shield RP18, 130 Å UHPLC: 2.5 μmXP HPLC: 3.5, 5, 10 μm	3.3	17%	Yes	L1	2-11	Low pH = 50 ˚C, High pH = 45 ˚C	185
BEH C8, 130 Å UHPLC: 2.5 μm XP HPLC: 3.5, 5, 10 μm	3.2	13%	Yes	L7	1-12	Low pH = 60 ˚C, High pH = 60 ˚C	185
BEH Phenyl, 130 Å UHPLC: 2.5 μm XP HPLC: 3.5, 5 μm	3.0	0,15	Yes	L11	1–12	Low pH = 80 ˚C, High pH = 60 ˚C	185
BEH HILIC, 130 Å UHPLC: 2.5 μm XP HPLC: 3.5 μm	N/A	Unbonded	Yes	L3	1–9	Low pH = 45 ˚C, High pH = 45 ˚C	185
BEH Amide, 130 Å UHPLC: 2.5 μm XP HPLC: 3.5 μm	7.5	0,12	No	L68	2–11	Low pH = 90 ˚C, High pH = 90 ˚C	185
Glycan BEH Amide, 130 Å UHPLC: 2.5 μm XP HPLC: 3.5 μm	7.5	0,12	No	L68	2–11	Low pH = 90 ˚C, High pH = 90 ˚C	185
Peptide BEH C18, 130 Å HPLC: 3.5, 5, 10 μm	3.1	0,18	Yes	L1	1–12	Low pH = 80 ˚C, High pH = 60 ˚C	185
Oligonucleotide BEH C18, 130 Å HPLC: 2.5 μm	3.1	0,18	Yes	L1	1–12	Low pH = 80 ˚C, High pH = 60 ˚C	90
Protein BEH C4, 300 Å HPLC: 3.5, 5, 10 μm	2.4	0,08	No	L26	1–10	Low pH = 80 ˚C, High pH = 50 ˚C	90
Protein BEH SEC, 125 Å HPLC: 3.5 μm	4.9	0,15	No	L33	1–8	Low pH = 60 ˚C, High pH = 60 ˚C	395
Protein BEH SEC, 200 Å HPLC: 3.5 μm	5.5	0,12	No	L33	1–8	Low pH = 60 ˚C, High pH = 60 ˚C	220
Protein BEH SEC, 450 Å HPLC: 3.5 μm	4.8	0,09	No	L33	1–8	Low pH = 60 ˚C, High pH = 60 ˚C	80

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Xbridge Protein BEH C4

Goals of Sample Preparation

Successful sample preparation for most analytical techniques (HPLC, UPLC, ™LC-MS, UV, GC, etc.)
has a threefold objective. It needs to provide the sample component of interest:
■■ In solution
■■ Free from interfering matrix elements
■■ At a concentration appropriate for detection or measurement

Waters™ Sample Preparation Solutions for quantitative analysis make it easy to deliver a sample that is reproducible with
high recovery and free of interferences. Based on simple, logical workflows that produce clean samples through selective
separations, Waters Sample Preparation Products maximize sensitivity, increase throughput, and enable the development of
robust methods.